sc-13974

antibody from Santa Cruz Biotechnology

Targeting: DVL2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-13974

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-13974, RRID:AB_639776

Product name

Anti-DVL2

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

IC261 induces cell cycle arrest and apoptosis of human cancer cells via CK1δ/ɛ and Wnt/β-catenin independent inhibition of mitotic spindle formation.

Cheong JK, Nguyen TH, Wang H, Tan P, Voorhoeve PM, Lee SH, Virshup DM
Oncogene 2011 Jun 2;30(22):2558-69

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The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling.

Chen M, Wang J, Lu J, Bond MC, Ren XR, Lyerly HK, Barak LS, Chen W
Biochemistry 2009 Nov 3;48(43):10267-74

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Placenta

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 79

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.