Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- sc-13974 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-13974, RRID:AB_639776
- Product name
- Anti-DVL2
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references IC261 induces cell cycle arrest and apoptosis of human cancer cells via CK1δ/ɛ and Wnt/β-catenin independent inhibition of mitotic spindle formation.
The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling.
Cheong JK, Nguyen TH, Wang H, Tan P, Voorhoeve PM, Lee SH, Virshup DM
Oncogene 2011 Jun 2;30(22):2558-69
Oncogene 2011 Jun 2;30(22):2558-69
The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling.
Chen M, Wang J, Lu J, Bond MC, Ren XR, Lyerly HK, Barak LS, Chen W
Biochemistry 2009 Nov 3;48(43):10267-74
Biochemistry 2009 Nov 3;48(43):10267-74
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Placenta
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 79
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.