PA5-71680

antibody from Invitrogen Antibodies

Targeting: SFTPC BRICD6, PSP-C, SFTP2, SMDP2, SP-C

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-71680

Provider

Invitrogen Antibodies

Product name

SFTPC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.45 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Prkg2 regulates alveolar type 2-mediated re-alveolarization.

Zhang M, Ali G, Komatsu S, Zhao R, Ji HL
Stem cell research & therapy 2022 Mar 21;13(1):111

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Interstitial lung disease in children with Rubinstein-Taybi syndrome.

Bradford L, Ross MK, Minso J, Cernelc-Kohan M, Shayan K, Wong SS, Li X, Rivier L, Jegga AG, Deutsch GH, Vece TJ, Loughlin CE, Gower WA, Hurley C, Furman W, Stokes D, Hagood JS
Pediatric pulmonology 2022 Jan;57(1):264-272

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Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.

Brookes O, Boland S, Lai Kuen R, Miremont D, Movassat J, Baeza-Squiban A
PloS one 2021;16(9):e0248798

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YAP and Wnt3a independently promote AECIIs proliferation and differentiation by increasing nuclear β‑catenin expression in experimental bronchopulmonary dysplasia.

Jia X, Wu B, Huang J, Fan L, Yang M, Xu W
International journal of molecular medicine 2021 Jan;47(1):195-206

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Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia.

Krishnamurthy S, Wohlford-Lenane C, Kandimalla S, Sartre G, Meyerholz DK, Théberge V, Hallée S, Duperré AM, Del'Guidice T, Lepetit-Stoffaes JP, Barbeau X, Guay D, McCray PB Jr
Nature communications 2019 Oct 28;10(1):4906

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SFTPC in human lung cells using confocal microscopy. Samples were incubated with SFTPC H1 polyclonal antibody (Product # PA5-71680) using a dilution of 1:25. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) with a dilution of 1:400 was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SFTPC in human lung cells using confocal microscopy. Samples were incubated with SFTPC H1 polyclonal antibody (Product # PA5-71680) using a dilution of 1:25. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) with a dilution of 1:400 was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 YAP, Wnt3a and beta-catenin expression is decreased in lung tissue affected by BPD. (A) YAP mRNA and (C) protein expression in the lungs. (B) Wnt3a mRNA and (C) protein expression in the lungs. (D) beta-catenin protein expression in lung nucleoli. Data are expressed as the means +- SD (n=6). * P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Effects of YAP on the proliferation and differentiation of AECIIs into AECIs. (A and B) SPC and AQP5 expression in OEYAP, KDYAP and corre-sponding control (OENC, KDNC) AECIIs detected by western blot analysis. Data are expressed as the means +- SD (n=3). * P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Wnt3a overexpression and knockdown reversed the effects of YAP on AECIIs proliferation and differentiation. (A) YAP expression in OEWnt3a, KDWnt3a and corresponding control (OENC, KDNC) AECIIs determined by western blot analysis. Data are expressed as the means +- SD (n=3). (B, D and E) SPC, AQP5 and nuclear beta-catenin expression detected by (B) western blot analysis and double IF staining for (D) SPC and beta-catenin or (E) AQP5 and beta-catenin in OEYAP, KDWnt3a, OEYAP + KDWnt3a, and corresponding control (OENC, KDNC) AECIIs extracted from hyperoxia-exposed rats after 14 days. Data are expressed as the means +- SD (n=3). * P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 Figure Immunofluorescence overlay images; x20; blue (DAPI): nuclei; Green (FITC): alpha-smooth muscle actin (aSMA); Red (AF568): surfactant protein C (SP-C); white (AF633): ATP binding cassete-A3 (ABCA3) (A) control; (B) Case 1; (C) Case 2; (D) Case 3. Green arrows: SP-C and ABCA3 colocalization in alveolar type 2 (AT2) pneumocytes; white arrows: aSMA staining around small vessels and airways; yellow arrows: aSMA+ interstitial myofibroblasts [Color figure can be viewed at wileyonlinelibrary.com ]