PA5-76631

antibody from Invitrogen Antibodies

Targeting: SFTPC BRICD6, PSP-C, SFTP2, SMDP2, SP-C

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-76631

Provider

Invitrogen Antibodies

Product name

SFTPC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Recessive missense LAMP3 variant associated with defect in lamellar body biogenesis and fatal neonatal interstitial lung disease in dogs.

Dillard KJ, Ochs M, Niskanen JE, Arumilli M, Donner J, Kyöstilä K, Hytönen MK, Anttila M, Lohi H
PLoS genetics 2020 Mar;16(3):e1008651

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 LAMP3, SP-B and SP-C immunohistochemistry. In the affected (A) and age matched 3-day-old control puppies (B), LAMP3 protein expression was detected as granular, globular to ring-like intracytoplasmic structures (red) in the AECII cells (100X, Z-stack, scale bar 20 mum). In the affected 3-day-old puppy (C), the proteinaceous material in the airspaces stained intensely with SP-B antibody (red), whereas in the age-matched control (D), SP-B positive staining was detected only in the cytoplasm of AECII cells and the epithelium of few bronchioles (10X, scale bar 200 mum). In the 28-day-old affected puppy (E), the significant increase of the SP-C positive cells (red) indicated AECII hyperplasia. In comparison, the age-matched control (F) had only scattered SP-C positive AECII cells in the alveolar epithelium (10X, scale bar 200 mum).