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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14076 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14076, RRID:AB_10549777
- Product name
- Fkbp5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Fkbp5.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Fkbp5 polyclonal antibody (Cat # PAB14076), diluted 1 : 500 in BSA/PBS-Tween.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of Fkbp5 polyclonal antibody (Cat # PAB14076).The plates were coated with a mix of the peptides used for immunization of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 9,400.
- Validation comment
- ELISA
