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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-15337 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-15337, RRID:AB_2150998
- Product name
- Anti-NEO1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Neogenin expression may be inversely correlated to the tumorigenicity of human breast cancer.
Lee JE, Kim HJ, Bae JY, Kim SW, Park JS, Shin HJ, Han W, Kim SW, Kang KS, Noh DY
BMC cancer 2005 Dec 3;5:154
BMC cancer 2005 Dec 3;5:154
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Supportive validation
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- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 160
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows distinct dot like cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
