Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1079 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Neogenin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Neogenin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DCC is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_032710
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of neogenin fosters resolution of inflammation and tissue regeneration.
Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells.
Tbr1 instructs laminar patterning of retinal ganglion cell dendrites.
Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats.
The axonal guidance receptor neogenin promotes acute inflammation.
Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates.
Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system.
Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin.
Schlegel M, Körner A, Kaussen T, Knausberg U, Gerber C, Hansmann G, Jónasdóttir HS, Giera M, Mirakaj V
The Journal of clinical investigation 2018 Oct 1;128(10):4711-4726
The Journal of clinical investigation 2018 Oct 1;128(10):4711-4726
Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells.
Hadi T, Boytard L, Silvestro M, Alebrahim D, Jacob S, Feinstein J, Barone K, Spiro W, Hutchison S, Simon R, Rateri D, Pinet F, Fenyo D, Adelman M, Moore KJ, Eltzschig HK, Daugherty A, Ramkhelawon B
Nature communications 2018 Nov 27;9(1):5022
Nature communications 2018 Nov 27;9(1):5022
Tbr1 instructs laminar patterning of retinal ganglion cell dendrites.
Liu J, Reggiani JDS, Laboulaye MA, Pandey S, Chen B, Rubenstein JLR, Krishnaswamy A, Sanes JR
Nature neuroscience 2018 May;21(5):659-670
Nature neuroscience 2018 May;21(5):659-670
Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats.
Frýdlová J, Přikryl P, Truksa J, Falke LL, Du X, Gurieva I, Vokurka M, Krijt J
PloS one 2016;11(2):e0148540
PloS one 2016;11(2):e0148540
The axonal guidance receptor neogenin promotes acute inflammation.
König K, Gatidou D, Granja T, Meier J, Rosenberger P, Mirakaj V
PloS one 2012;7(3):e32145
PloS one 2012;7(3):e32145
Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates.
Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jan 11;32(2):411-6
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jan 11;32(2):411-6
Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
The American journal of pathology 2009 Sep;175(3):1010-8
The American journal of pathology 2009 Sep;175(3):1010-8
Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system.
Osterfield M, Egelund R, Young LM, Flanagan JG
Development (Cambridge, England) 2008 Mar;135(6):1189-99
Development (Cambridge, England) 2008 Mar;135(6):1189-99
Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
Lejmi E, Leconte L, Pédron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouët J
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 26;105(34):12491-6
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 26;105(34):12491-6
Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin.
Kuns-Hashimoto R, Kuninger D, Nili M, Rotwein P
American journal of physiology. Cell physiology 2008 Apr;294(4):C994-C1003
American journal of physiology. Cell physiology 2008 Apr;294(4):C994-C1003
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Neogenin by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, Jurkat human acute T cell leukemia cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neogenin at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Neogenin by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neogenin at approximately 207 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Neogenin in Mouse Embryo. Neogenin was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.