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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANR-195-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neogenin (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 2 and 4): - 1-2. Anti-Neogenin (extracellular) Antibody (#ANR-195), (1:200). 3-4. Anti-Neogenin (extracellular) Antibody , preincubated with Neogenin (extracellular) Blocking Peptide (BLP-NR195).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Neogenin in rat parietal cortex. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neogenin (extracellular) Antibody (#ANR-195), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Neogenin immunoreactivity (green) appears in cortical neurons in layer 3 (arrows). B. Pre-incubation of the Antibody with Neogenin (extracellular) Blocking Peptide (BLP-NR195), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Neogenin by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Neogenin (extracellular) Antibody (#ANR-195) (2.5μg) + goat- Anti-rabbit-FITC.
