Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-9019 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BLNK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of BLNK in Daudi lysate using Product # PA1-9019.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BLNK in Daudi cell lysate using a BLNK polyclonal antibody (Product # PA1-9019) at a concentration of 0.3 µg/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BLNK in HepG2 cells using a BLNK polyclonal antibody (Product # PA1-9019) at a concentration of 10 µg/mL. The sample was paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation for 1hr was followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BLNK in HepG2 cells using a BLNK polyclonal antibody (Product # PA1-9019) at a concentration of 10 µg/mL. The sample was paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation for 1hr was followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of BLNK in Daudi cells using a polyclonal antibody (Product #PA1-9019). Daudi cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
