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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 11708-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11708-1-AP, RRID:AB_2255114
- Product name
- SMN antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SMN antibody (Cat. #11708-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Dual SMN inducing therapies can rescue survival and motor unit function in symptomatic ∆7SMA mice.
Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.
Intragenic complementation of amino and carboxy terminal SMN missense mutations can rescue Smn null mice.
Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.
SMN Protein Can Be Reliably Measured in Whole Blood with an Electrochemiluminescence (ECL) Immunoassay: Implications for Clinical Trials.
Baseline results of the NeuroNEXT spinal muscular atrophy infant biomarker study.
SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.
Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.
Detection of survival motor neuron protein in buccal cells through electrochemiluminescence-based assay.
Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.
Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.
Kray KM, McGovern VL, Chugh D, Arnold WD, Burghes AHM
Neurobiology of disease 2021 Nov;159:105488
Neurobiology of disease 2021 Nov;159:105488
Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.
Zhang Y, Chen X, Wang Q, Du C, Lu W, Yuan H, Zhang Z, Li D, Ling X, Ren X, Zhao Y, Su Q, Xing Z, Qin Y, Yang X, Shen Y, Wu H, Qi Y
Journal of molecular medicine (Berlin, Germany) 2021 Dec;99(12):1797-1813
Journal of molecular medicine (Berlin, Germany) 2021 Dec;99(12):1797-1813
Intragenic complementation of amino and carboxy terminal SMN missense mutations can rescue Smn null mice.
McGovern VL, Kray KM, Arnold WD, Duque SI, Iyer CC, Massoni-Laporte A, Workman E, Patel A, Battle DJ, Burghes AHM
Human molecular genetics 2020 Nov 1;29(21):3493-3503
Human molecular genetics 2020 Nov 1;29(21):3493-3503
Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.
Iyer CC, Corlett KM, Massoni-Laporte A, Duque SI, Madabusi N, Tisdale S, McGovern VL, Le TT, Zaworski PG, Arnold WD, Pellizzoni L, Burghes AHM
Human molecular genetics 2018 Oct 1;27(19):3404-3416
Human molecular genetics 2018 Oct 1;27(19):3404-3416
SMN Protein Can Be Reliably Measured in Whole Blood with an Electrochemiluminescence (ECL) Immunoassay: Implications for Clinical Trials.
Zaworski P, von Herrmann KM, Taylor S, Sunshine SS, McCarthy K, Risher N, Newcomb T, Weetall M, Prior TW, Swoboda KJ, Chen KS, Paushkin S
PloS one 2016;11(3):e0150640
PloS one 2016;11(3):e0150640
Baseline results of the NeuroNEXT spinal muscular atrophy infant biomarker study.
Kolb SJ, Coffey CS, Yankey JW, Krosschell K, Arnold WD, Rutkove SB, Swoboda KJ, Reyna SP, Sakonju A, Darras BT, Shell R, Kuntz N, Castro D, Iannaccone ST, Parsons J, Connolly AM, Chiriboga CA, McDonald C, Burnette WB, Werner K, Thangarajh M, Shieh PB, Finanger E, Cudkowicz ME, McGovern MM, McNeil DE, Finkel R, Kaye E, Kingsley A, Renusch SR, McGovern VL, Wang X, Zaworski PG, Prior TW, Burghes AH, Bartlett A, Kissel JT, NeuroNEXT Clinical Trial Network and on behalf of the NN101 SMA Biomarker Investigators.
Annals of clinical and translational neurology 2016 Feb;3(2):132-45
Annals of clinical and translational neurology 2016 Feb;3(2):132-45
SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.
McGovern VL, Iyer CC, Arnold WD, Gombash SE, Zaworski PG, Blatnik AJ 3rd, Foust KD, Burghes AH
Human molecular genetics 2015 Oct 1;24(19):5524-41
Human molecular genetics 2015 Oct 1;24(19):5524-41
Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.
Iyer CC, McGovern VL, Murray JD, Gombash SE, Zaworski PG, Foust KD, Janssen PM, Burghes AH
Human molecular genetics 2015 Nov 1;24(21):6160-73
Human molecular genetics 2015 Nov 1;24(21):6160-73
Detection of survival motor neuron protein in buccal cells through electrochemiluminescence-based assay.
Steinkellner H, Etzler J, Gmeiner BM, Laccone F
Assay and drug development technologies 2015 Apr;13(3):167-73
Assay and drug development technologies 2015 Apr;13(3):167-73
Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.
Crawford TO, Paushkin SV, Kobayashi DT, Forrest SJ, Joyce CL, Finkel RS, Kaufmann P, Swoboda KJ, Tiziano D, Lomastro R, Li RH, Trachtenberg FL, Plasterer T, Chen KS, Pilot Study of Biomarkers for Spinal Muscular Atrophy Trial Group.
PloS one 2012;7(4):e33572
PloS one 2012;7(4):e33572
Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.
Kobayashi DT, Olson RJ, Sly L, Swanson CJ, Chung B, Naryshkin N, Narasimhan J, Bhattacharyya A, Mullenix M, Chen KS
PloS one 2011;6(8):e24269
PloS one 2011;6(8):e24269
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP(SMN2 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SMN2 antibody 11708-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP result of anti-SMN2(11708-1-AP for IP and Detection).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 11708-1-AP(SMN2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 11708-1-AP(SMN2 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
