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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-15325 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-15325, RRID:AB_634092
- Product name
- Anti-ARC
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Selective survival and maturation of adult-born dentate granule cells expressing the immediate early gene Arc/Arg3.1.
Object-place recognition learning triggers rapid induction of plasticity-related immediate early genes and synaptic proteins in the rat dentate gyrus.
The neuroplasticity-associated arc gene is a direct transcriptional target of early growth response (Egr) transcription factors.
Kuipers SD, Tiron A, Soule J, Messaoudi E, Trentani A, Bramham CR
PloS one 2009;4(3):e4885
PloS one 2009;4(3):e4885
Object-place recognition learning triggers rapid induction of plasticity-related immediate early genes and synaptic proteins in the rat dentate gyrus.
Soulé J, Penke Z, Kanhema T, Alme MN, Laroche S, Bramham CR
Neural plasticity 2008;2008:269097
Neural plasticity 2008;2008:269097
The neuroplasticity-associated arc gene is a direct transcriptional target of early growth response (Egr) transcription factors.
Li L, Carter J, Gao X, Whitehead J, Tourtellotte WG
Molecular and cellular biology 2005 Dec;25(23):10286-300
Molecular and cellular biology 2005 Dec;25(23):10286-300
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 45
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
