Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-22117 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal YAP1 Antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references YAP1 and AR interactions contribute to the switch from androgen-dependent to castration-resistant growth in prostate cancer.
Kuser-Abali G, Alptekin A, Lewis M, Garraway IP, Cinar B
Nature communications 2015 Sep 1;6:8126
Nature communications 2015 Sep 1;6:8126
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Analysis using YAP1 mAb against human YAP1 (AA: 250-447) recombinant protein. (Expected MW is 54.4 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate. Dilution 1:500 - 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: YAP1 Antibody (1A12) [NBP2-22117] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng). Dilution 1:10000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded rectum cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red). Dilution 1:200 - 1:400.