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- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-17200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- YAP1 Monoclonal Antibody (1A12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17200 targets YAP1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-17200 immunogen is purified recombinant fragment of human YAP1 expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A12
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of YAP1 using a YAP1 monoclonal antibody (Product # MA5-17200) against a human YAP1 (AA: 250-447) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of histone YAP was performed by loading 40 µg of MCF-7 cell lysates and 3 µL PageRuler Prestained Protein Ladder (Product # 26619) per well onto a 10% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk/PBS-T for 1 h at room temperature. YAP1 was detected using a YAP1 antibody (Product # MA5-17200) at 1:1000 dilution in 5% BSA/PBS-T buffer overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody at a dilution in 5% skim milk/PBS-T of 1:3000 for 2 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Data courtesy of Dr. Wei Xu at the University of Wisconsin, Madison, WI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of YAP1 using YAP1 monoclonal antibody (Product # MA5-17200) in HeLa (1), C6 (2) and COS-7 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of YAP1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR772157_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of YAP1 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa YAP1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-YAP1 Monoclonal Antibody (1A12) (Product # MA5-17200, 1:2,000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to YAP1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK293 (Lane 1), HeLa (Lane 2), U-2 OS (Lane 3), MDA-MB-231 (Lane 4), SK-BR-3 (Lane 5), MCF-7 (Lane 6), Jurkat (Lane 7) and Raji (Lane 8). The blot was probed with Anti-YAP1 Monoclonal Antibody (Product # MA5-17200, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 62 kDa band corresponding to YAP1 was observed across all the cell lines tested except Jurkat and Raji.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of YAP1 using a YAP1 monoclonal antibody (Product # MA5-17200) against a human YAP1 (AA: 250-447) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of YAP1 using YAP1 monoclonal antibody (Product # MA5-17200) in HeLa (1), C6 (2) and COS-7 (3) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using YAP1 monoclonal antibody (Product # MA5-17200) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using YAP1 monoclonal antibody (Product # MA5-17200) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using YAP1 monoclonal antibody (Product # MA5-17200) (green) and negative control (red).
