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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX130444 - Provider product page
- Provider
- GeneTex
- Product name
- YAP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Zebrafish
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- YAP1 antibody detects YAP1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of YAP1 protein from HeLa whole cell extracts using 5 £gg of YAP1 antibody (GTX130444).Western blot analysis was performed using YAP1 antibody (GTX130444).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
