H00010413-M03

antibody from Abnova Corporation

Targeting: YAP1 YAP65

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010413-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010413-M03, RRID:AB_626507

Product name

YAP1 monoclonal antibody (M03), clone 2H1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant YAP1.

Antigen sequence

QIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMR
LRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQH
VRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTA
QHLR


Isotype

IgG

Antibody clone number

2H1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

YAP1 monoclonal antibody (M03), clone 2H1 Western Blot analysis of YAP1 expression in Hela S3 NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged YAP1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell. [antibody concentration 40 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol