Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9967 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-YAP1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for YAP/YAP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. YAP/YAP1 information: Molecular Weight: 54462 MW; Subcellular Localization: Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271); Tissue Specificity: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of YAP1 expression in rat testis extract (lane 1), mouse ovary extract (lane 2), HELA whole cell lysates (lane 3) and MCF-7 whole cell lysates (lane 4). YAP1 at 54KD; 70KD was detected using rabbit anti-YAP1 Antigen Affinity purified polyclonal antibody (Catalog # PB9967) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image