Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- P00116 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for YAP/YAP1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human, Mouse, Rat YAP/YAP1 information: Molecular Weight: 54462 MW; Subcellular Localization: Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271); Tissue Specificity: Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- GAB-25
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-YAP1 (S127) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with FBS+calyculin A.
- Additional image