Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-17481 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-YAP1 (Ser127) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 59.2 µg/mL
- Storage
- -20°C
Submitted references The aryl hydrocarbon receptor promotes differentiation during mouse preimplantational embryo development.
Livin promotes tumor progression through YAP activation in ovarian cancer.
FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.
Nacarino-Palma A, González-Rico FJ, Rejano-Gordillo CM, Ordiales-Talavero A, Merino JM, Fernández-Salguero PM
Stem cell reports 2021 Sep 14;16(9):2351-2363
Stem cell reports 2021 Sep 14;16(9):2351-2363
Livin promotes tumor progression through YAP activation in ovarian cancer.
Gao J, Han W, He Y, Zhou J, Miao J, Zhang G
American journal of cancer research 2020;10(10):3179-3193
American journal of cancer research 2020;10(10):3179-3193
FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.
Malgundkar SH, Burney I, Al Moundhri M, Al Kalbani M, Lakhtakia R, Okamoto A, Tamimi Y
BMC cancer 2020 May 4;20(1):374
BMC cancer 2020 May 4;20(1):374
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with Forskolin (10uM for 60 min) (Lane 2), PC-3 (Lane 3), PC-3 treated with Forskolin (10uM for 60 min) and U-87 MG (Lane 5). The blot was probed with Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody (Product # PA5-17481, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). A 65 kDa band corresponding to Phospho-YAP1 (Ser127) was observed across cell lines tested and was enhanced upon Forskolin treatment.