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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-17609 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- YAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 31 µg/mL
- Storage
- -20°C
Submitted references The hippo pathway is activated and is a causal mechanism for adipogenesis in arrhythmogenic cardiomyopathy.
Chen SN, Gurha P, Lombardi R, Ruggiero A, Willerson JT, Marian AJ
Circulation research 2014 Jan 31;114(3):454-68
Circulation research 2014 Jan 31;114(3):454-68
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK293 (Lane 1), HeLa (Lane 2), U-2 OS (Lane 3), MDA-MB-231 (Lane 4), SK-BR-3 (Lane 5), MCF-7 (Lane 6), Jurkat (Lane 7) and Raji (Lane 8). The blot was probed with Anti-YAP1 Polyclonal Antibody (Product # PA5-17609, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 62 kDa band corresponding to YAP1 was observed across all the cell lines tested except Jurkat and Raji.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
