Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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Validation data
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- Product number
- 21470-1 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IFN-beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Binds to human interferon beta with high affinity
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- After receipt, the product may be stored at -20degreesC for short-term use (= 6 months). For long-term storage, we recommend storing the product at -70degreesC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- ELISA: IFN-beta Antibody [21470-1] - Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 ug/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3, 3', 5, 5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.