21460-1

antibody from Novus Biologicals

Targeting: IFNB1 IFB, IFF, IFNB

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

21460-1

Provider

Novus Biologicals

Product name

Mouse Monoclonal IFN-beta Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Binds to human interferon beta with high affinity

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Storage

After receipt, the product may be stored at -20degreesC for short-term use (= 6 months). For long-term storage, we recommend storing the product at -70degreesC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: IFN-beta Antibody [21460-1] - Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 ug/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3, 3', 5, 5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.