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- Antibody Data
- Antigen structure
- References [3]
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- ELISA [1]
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- Product number
- 21450-1 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IFN-beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human interferon beta in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- After receipt, the product may be stored at -20degreesC for short-term use ( = 6 months). For long-term storage, we recommend storing the product at -70degreesC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Submitted references TLR7 agonist 852A inhibition of tumor cell proliferation is dependent on plasmacytoid dendritic cells and type I IFN.
Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
Inglefield JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2008 Apr;28(4):253-63
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2008 Apr;28(4):253-63
Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
Moll HP, Freudenthaler H, Zommer A, Buchberger E, Brostjan C
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 15;180(8):5250-6
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 15;180(8):5250-6
Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
Moll HP, Freudenthaler H, Zommer A, Buchberger E, Brostjan C
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 15;180(8):5250-6
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 15;180(8):5250-6
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- ELISA: IFN-beta Antibody [21450-1] - Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 ug/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3, 3', 5, 5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.
