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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- ELISA [1]
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- Product number
- 500-P32B-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFN beta Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-beta. Anti-Human IFN-beta-specific antibody was purified by affinity chromatography employing an immobilized Human IFN-beta matrix. Sandwich ELISA: To detect Human IFN-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IFN-beta (500-P32BBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-beta. Western Blot: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references PARP7 negatively regulates the type I interferon response in cancer cells and its inhibition triggers antitumor immunity.
Respiratory infections regulated blood cells IFN-β-PD-L1 pathway in pediatric asthma.
STAT3/p53 pathway activation disrupts IFN-β-induced dormancy in tumor-repopulating cells.
Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function.
The oncolytic virus dl922-947 reduces IL-8/CXCL8 and MCP-1/CCL2 expression and impairs angiogenesis and macrophage infiltration in anaplastic thyroid carcinoma.
Type I interferon induces CX3CL1 (fractalkine) and CCL5 (RANTES) production in human pulmonary vascular endothelial cells.
Gozgit JM, Vasbinder MM, Abo RP, Kunii K, Kuplast-Barr KG, Gui B, Lu AZ, Molina JR, Minissale E, Swinger KK, Wigle TJ, Blackwell DJ, Majer CR, Ren Y, Niepel M, Varsamis ZA, Nayak SP, Bamberg E, Mo JR, Church WD, Mady ASA, Song J, Utley L, Rao PE, Mitchison TJ, Kuntz KW, Richon VM, Keilhack H
Cancer cell 2021 Sep 13;39(9):1214-1226.e10
Cancer cell 2021 Sep 13;39(9):1214-1226.e10
Respiratory infections regulated blood cells IFN-β-PD-L1 pathway in pediatric asthma.
Kölle J, Haag P, Vuorinen T, Alexander K, Rauh M, Zimmermann T, Papadopoulos NG, Finotto S
Immunity, inflammation and disease 2020 Sep;8(3):310-319
Immunity, inflammation and disease 2020 Sep;8(3):310-319
STAT3/p53 pathway activation disrupts IFN-β-induced dormancy in tumor-repopulating cells.
Liu Y, Lv J, Liu J, Liang X, Jin X, Xie J, Zhang L, Chen D, Fiskesund R, Tang K, Ma J, Zhang H, Dong W, Mo S, Zhang T, Cheng F, Zhou Y, Jia Q, Zhu B, Kong Y, Guo J, Zhang H, Hu ZW, Cao X, Qin FX, Huang B
The Journal of clinical investigation 2018 Mar 1;128(3):1057-1073
The Journal of clinical investigation 2018 Mar 1;128(3):1057-1073
Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function.
Petri RM, Hackel A, Hahnel K, Dumitru CA, Bruderek K, Flohe SB, Paschen A, Lang S, Brandau S
Stem cell reports 2017 Sep 12;9(3):985-998
Stem cell reports 2017 Sep 12;9(3):985-998
The oncolytic virus dl922-947 reduces IL-8/CXCL8 and MCP-1/CCL2 expression and impairs angiogenesis and macrophage infiltration in anaplastic thyroid carcinoma.
Passaro C, Borriello F, Vastolo V, Di Somma S, Scamardella E, Gigantino V, Franco R, Marone G, Portella G
Oncotarget 2016 Jan 12;7(2):1500-15
Oncotarget 2016 Jan 12;7(2):1500-15
Type I interferon induces CX3CL1 (fractalkine) and CCL5 (RANTES) production in human pulmonary vascular endothelial cells.
Nakano M, Fujii T, Hashimoto M, Yukawa N, Yoshifuji H, Ohmura K, Nakaizumi A, Mimori T
Clinical and experimental immunology 2012 Oct;170(1):94-100
Clinical and experimental immunology 2012 Oct;170(1):94-100
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human IFN-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IFN beta Polyclonal Antibody (Product # 500-P32B-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IFN beta Polyclonal Antibody, Biotin (Product # 500-P32BBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-beta.
