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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF814 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-beta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IFN-beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IFN-beta 1, recombinant human IFN-gamma , recombinant cotton rat (rcr) IFN-alpha and rcrIFN-gamma is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans.
Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-γ.
The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes.
TLR3 can directly trigger apoptosis in human cancer cells.
TLR3 can directly trigger apoptosis in human cancer cells.
Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Krüger DH, Klempa B
PloS one 2012;7(4):e35587
PloS one 2012;7(4):e35587
Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-γ.
Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
PLoS pathogens 2011 Apr;7(4):e1002016
PLoS pathogens 2011 Apr;7(4):e1002016
The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes.
Rascle A, Neumann T, Raschta AS, Neumann A, Heining E, Kastner J, Witzgall R
Experimental cell research 2009 Jan 1;315(1):76-96
Experimental cell research 2009 Jan 1;315(1):76-96
TLR3 can directly trigger apoptosis in human cancer cells.
Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4894-901
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4894-901
TLR3 can directly trigger apoptosis in human cancer cells.
Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4894-901
Journal of immunology (Baltimore, Md. : 1950) 2006 Apr 15;176(8):4894-901
Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
The Journal of general virology 2004 Sep;85(Pt 9):2603-13
The Journal of general virology 2004 Sep;85(Pt 9):2603-13
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-beta Antibody. Recombinant Human IFN-beta (Catalog # 8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND50 is typically 0.05-0.2 µg/mL.
