Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-77303 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20°C
Submitted references Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells.
Furin Prodomain ppFurin Enhances Ca(2+) Entry Through Orai and TRPC6 Channels' Activation in Breast Cancer Cells.
Sanchez-Collado J, Lopez JJ, Jardin I, Berna-Erro A, Camello PJ, Cantonero C, Smani T, Salido GM, Rosado JA
Cellular and molecular life sciences : CMLS 2022 Jan 6;79(1):33
Cellular and molecular life sciences : CMLS 2022 Jan 6;79(1):33
Furin Prodomain ppFurin Enhances Ca(2+) Entry Through Orai and TRPC6 Channels' Activation in Breast Cancer Cells.
López JJ, Siegfried G, Cantonero C, Soulet F, Descarpentrie J, Smani T, Badiola I, Pernot S, Evrard S, Rosado JA, Khatib AM
Cancers 2021 Apr 1;13(7)
Cancers 2021 Apr 1;13(7)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes with TRPC1 polyclonal antibody (Product # PA5-77303) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPC1 in frozen mouse cerebellum. A) Samples were probed with TRPC1 polyclonal antibody (Product # PA5-77303) and incubated with parvalbumin. B) Stains of brain section. C) Confocal merged image.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ppFurin enhances store-operated Ca 2+ entry (SOCE) in MDA-MB-231 cells. ( a ) Control MDA-MB-231 and MDA-MB-435 cells or expressing ppFurin were lysed and whole cell lysates were analyzed by Western blotting using anti-Orai1, Orai3, STIM1, STIM2, TRPC1, TRPC6, or beta-actin antibody, as indicated. Blot images are from one experiment representative of three which gave similar results. ( b - e ). Changes in [Ca 2+ ] I were detected in fura-2-loaded control MDA-MB-231 and MDA-MB-435 cells or expressing ppFurin. Cells were stimulated with 1 uM of TG in a Ca 2+ -free medium (100 uM of EGTA added), and later SOCE was detected by the addition of 1 mM of CaCl 2 to the extracellular medium. ( b , c ) Traces are representative of 40 cells/day/3-5 days. ( d , e ) Histograms indicate TG-induced Ca 2+ release and SOCE as the area under the curve, expressed as mean +- SEM and presented as percentage of their respective control cells. Statistical significance was assessed by Student''s t -test and * represents p < 0.05 as compared to cells transfected with pIRES2-EGFP empty vector.