ANT-201-200UL

antibody from Invitrogen Antibodies

Targeting: SLC11A1 LSH, NRAMP, NRAMP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANT-201-200UL

Provider

Invitrogen Antibodies

Product name

SLC11A1/NRAMP1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat spleen lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 4) and mouse lung membranes (lanes 3 and 6): - 1-3. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200). 4-6. Anti-SLC11A1/NRAMP1 (extracellular) Antibody , preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human THP-1 monocytic leukemia cells: - 1. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200) 2. Anti-SLC11A1/NRAMP1 (extracellular) Antibody , preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NRAMP1 in rat hippocampus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:600), followed by goat Anti-rabbit-Alexa-488. A. Staining in the dentate gyrus region showed NRAMP1 immunoreactivity (green) in the granule layer (G) and in hilar interneurons (arrows). B. Pre-incubation of the Antibody with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201), suppressed staining. Cell nuclei are stained with DAPI (blue). H = hilus.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of SLC11A1 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (5 µg) + goat- Anti-rabbit-FITC.