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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [3]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C197177 - Provider product page
- Provider
- LSBio
- Product name
- F3 / CD142 / Tissue factor Antibody (clone 4G4) LS-C197177
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4G4
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- F3 monoclonal antibody (M01), clone 4G4 Western Blot analysis of F3 expression in A-431.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- F3 monoclonal antibody (M01), clone 4G4. Western Blot analysis of F3 expression in Jurkat.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody (M01), clone 4G4.Lane 1: F3 transfected lysate(33.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged F3 is approximately 0.03 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged F3 is approximately 0.03 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged F3 is approximately 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of F3 transfected lysate using anti-F3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F3 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of F3 transfected lysate using anti-F3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F3 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of F3 transfected lysate using anti-F3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F3 rabbit polyclonal antibody.
