MA1-43029

antibody from Invitrogen Antibodies

Targeting: F3 CD142, TF

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-43029

Provider

Invitrogen Antibodies

Product name

CD142 Monoclonal Antibody (5264)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-43029 detects Tissue Factor in human samples. MA1-43029 has successfully been used in Western blot and ELISA applications. MA1-43029 was derived from human tissue factor.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5264

Vial size

100 µg

Concentration

4.7 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD142 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD142 Monoclonal Antibody (5264) (Product # MA1-43029) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane and cytoplasmic localization in MDA-MB-231 cells but not in MCF7 cells (Panel e), which is reported to be negative for CD142 expression. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.