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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB2339-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Coagulation Factor III/Tissue Factor Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human coagulation factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Tissue Factor is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P13726- Isotype
- IgG
- Antibody clone number
- 323514
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
Méndez-Cruz AR, Paez A, Jiménez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, López-Marure R, Masso FA, Flores-Romo L, Montaño LF
Immunology letters 2007 Aug 15;111(2):116-23
Immunology letters 2007 Aug 15;111(2):116-23
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Coagulation Factor III by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line treated (+) with TNF-alpha . PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB2339) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Coagulation Factor III at approximately 50 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
