F52225

antibody from NSJ Bioreagents

Targeting: TBP GTF2D1, SCA17, TFIID

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F52225

Provider

NSJ Bioreagents

Product name

TBP Antibody / TATA-box-binding protein

Antibody type

Monoclonal

Description

This highly specific TBP antibody is suitable for use in Immunohistochemistry/Western blot/ELISA applications with human, mouse and rat, monkey samples.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

830CT4.3.3

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human 293, 2) (h) HeLa, 3) (h) MDA-MB-231, 4) rat H-4-II-E, 5) (h) HCT-116 and 6) mouse C2C12 cell lysate with TBP antibody at 1:2000. Expected molecular weight: 35-43 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

TBP antibody western blot analysis in (1) human HeLa, (2) (h) HepG2, (3) mouse NIH3T3 lysate. Expected molecular weight: 35-43 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human testis using TBP antibody at 1:25 dilution.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human testis tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE monkey brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.