Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- LS-C150111 - Provider product page
- Provider
- LSBio
- Product name
- Anti-GLI / GLI1 Antibody (clone 388516) LS-C150111
- Antibody type
- Monoclonal
- Description
- Protein A/G purified
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 388516
- Vial size
- 100µg
- Storage
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human/Mouse GLI-1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 ug/ml of Human/Mouse GLI-1 Monoclonal Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for GLI-1 at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- GLI-1 in A1 72 Human Cell Line. GLI-1 was detected in immersion fixed A172 human glioblastoma cell line using 10 ug/ml Human/Mouse GLI-1 Monoclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel).