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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA803697 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA803697, RRID:AB_2626958
- Product name
- Purified GLI1 mouse monoclonal antibody, clone OTI2D5E2
- Antibody type
- Monoclonal
- Description
- Purified GLI1 mouse monoclonal antibody, clone OTI2D5E2
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GLI1
- Isotype
- IgG
- Antibody clone number
- OTI2D5E2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803697)
- Validation comment
- IHC
