TA803969

antibody from OriGene

Targeting: GLI1 GLI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA803969

Provider

OriGene

Proper citation

OriGene Cat#TA803969, RRID:AB_2627067

Product name

GLI1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)

Antibody type

Monoclonal

Description

GLI1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)

Host

Mouse

Conjugate

Unconjugated

Epitope

GLI1

Isotype

IgG

Antibody clone number

OTI2E1

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803969)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803969)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803969)

Validation comment

IHC