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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [3]
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- Product number
- GTX124274 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX124274, RRID:AB_11174802
- Product name
- Gli1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma.
Wang K, Chen D, Qian Z, Cui D, Gao L, Lou M
Cancer cell international 2017;17:117
Cancer cell international 2017;17:117
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 5% SDS PAGE GTX124274 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Gli1 antibody detects Gli1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX124274) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX124274) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
