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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-23411 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLI1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Metformin alleviates hyperglycemia-induced endothelial impairment by downregulating autophagy via the Hedgehog pathway.
Polymeric glabrescione B nanocapsules for passive targeting of Hedgehog-dependent tumor therapy in vitro.
Niu C, Chen Z, Kim KT, Sun J, Xue M, Chen G, Li S, Shen Y, Zhu Z, Wang X, Liang J, Jiang C, Cong W, Jin L, Li X
Autophagy 2019 May;15(5):843-870
Autophagy 2019 May;15(5):843-870
Polymeric glabrescione B nanocapsules for passive targeting of Hedgehog-dependent tumor therapy in vitro.
Ingallina C, Costa PM, Ghirga F, Klippstein R, Wang JT, Berardozzi S, Hodgins N, Infante P, Pollard SM, Botta B, Al-Jamal KT
Nanomedicine (London, England) 2017 Apr;12(7):711-728
Nanomedicine (London, England) 2017 Apr;12(7):711-728
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLI1 in human kidney lysate. Samples were incubated in GLI1 polyclonal antibody (Product # PA5-23411) using a dilution of 1 µg/mL followed by a goat anti-rabbit IgG HRP secondary antibody. 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 µg/mL. PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GLI1 in C666-1 human nasopharyngel carcinoma cell line and OKF6 tert-1 human oral epithelial cell line. Samples were incubated in GLI1 polyclonal antibody (Product # PA5-23411) using a dilution of 10 µg/mL. Top panel: C666-1 malignant human nasopharyngel carcinoma cell line. Bottom panel: OKF6 tert-1 human oral epithelial cell line. GLI-1 was more highly expressed in the C666-1 than in the OKF6 tert-1 cells. See application notes for protocol details.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLI1 in formalin-fixed, paraffin-embedded human brain tissue. Samples were incubated in GLI1 polyclonal antibody (Product # PA5-23411) using a dilution of 5 µg/mL. Isotype control antibody (top) and GLI-1 antibody (bottom).
