H00001787-M01

antibody from Abnova Corporation

Targeting: TRDMT1 DNMT2, RNMT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001787-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001787-M01, RRID:AB_463886

Product name

DNMT2 monoclonal antibody (M01), clone 1E12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant DNMT2.

Antigen sequence

MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDV
NTVANEVYKYNFHHTQLLAKTIEGITLEEFDRLSF
DMILMSPPCQPFTRIGRQGDMTDSRTNSFLYILDI
LPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENC
GFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLP
FQAPGQVLMEFPKIESVHPQKYAMDVEEKIQEKNV
EPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQD
SDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLL
DIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQ
VENIYKSLTNLSQEEQITKLLILKLRYFTPKEIAN
LLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKL
IKILYE


Isotype

IgG

Antibody clone number

1E12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DNMT2 monoclonal antibody (M01), clone 1E12 Western Blot analysis of DNMT2 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DNMT2 expression in transfected 293T cell line by DNMT2 monoclonal antibody (M01), clone 1E12.Lane 1: DNMT2 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to DNMT2 on HeLa cell. [antibody concentration 40 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol