Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2186 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2186, RRID:AB_1579928
- Product name
- TRDMT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TRDMT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.
Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase 2 in human peripheral blood leukocytes.
Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA.
Hermann A, Schmitt S, Jeltsch A
The Journal of biological chemistry 2003 Aug 22;278(34):31717-21
The Journal of biological chemistry 2003 Aug 22;278(34):31717-21
Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase 2 in human peripheral blood leukocytes.
Franchina M, Hooper J, Kay PH
The international journal of biochemistry & cell biology 2001 Nov;33(11):1104-15
The international journal of biochemistry & cell biology 2001 Nov;33(11):1104-15
Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA.
Dong A, Yoder JA, Zhang X, Zhou L, Bestor TH, Cheng X
Nucleic acids research 2001 Jan 15;29(2):439-48
Nucleic acids research 2001 Jan 15;29(2):439-48
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TRDMT1 polyclonal antibody (Cat # PAB2186) in Jurkat cell lysate (Lane A) and mouse liver tissue lysate (Lane B) . Dnmt2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)