PAB2206

antibody from Abnova Corporation

Targeting: DNMT3A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2206

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2206, RRID:AB_1574430

Product name

DNMT3A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of DNMT3A.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors.

Robertson KD, Uzvolgyi E, Liang G, Talmadge C, Sumegi J, Gonzales FA, Jones PA
Nucleic acids research 1999 Jun 1;27(11):2291-8

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Cloning, expression and chromosome locations of the human DNMT3 gene family.

Xie S, Wang Z, Okano M, Nogami M, Li Y, He WW, Okumura K, Li E
Gene 1999 Aug 5;236(1):87-95

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of DNMT3A polyclonal antibody (Cat # PAB2206) in Jurkat cell line lysates (35 ug/lane). DNMT3A (arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinomareacted with DNMT3A polyclonal antibody (Cat # PAB2206), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)