Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00008493-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008493-M01, RRID:AB_606821
- Product name
- PPM1D monoclonal antibody (M01), clone 4D1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PPM1D.
- Antigen sequence
IGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTP
PTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPAS
LPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRK
TVCVC- Isotype
- IgG
- Antibody clone number
- 4D1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PPM1D expression in transfected 293T cell line by PPM1D monoclonal antibody (M01), clone 4D1.Lane 1: PPM1D transfected lysate(66.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PPM1D is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol