sc-20781

antibody from Santa Cruz Biotechnology

Targeting: CYBA p22-PHOX

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-20781

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-20781, RRID:AB_2090309

Product name

Anti-CYBA

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Germline CYBB mutations that selectively affect macrophages in kindreds with X-linked predisposition to tuberculous mycobacterial disease

Jacinta Bustamante, Andres A Arias, Guillaume Vogt, Capucine Picard, Lizbeth Blancas Galicia, Carolina Prando, Audrey V Grant, Christophe C Marchal, Marjorie Hubeau, Ariane Chapgier, Ludovic de Beaucoudrey, Anne Puel, Jacqueline Feinberg, Ethan Valinetz, Lucile Jannière, Céline Besse, Anne Boland, Jean-Marie Brisseau, Stéphane Blanche, Olivier Lortholary, Claire Fieschi, Jean-François Emile, Stéphanie Boisson-Dupuis, Saleh Al-Muhsen, Bruce Woda, Peter E Newburger, Antonio Condino-Neto, Mary C Dinauer, Laurent Abel, Jean-Laurent Casanova
Nature Immunology 2011 Jan;12(3):213-221

---

Molecular and functional characterization of Hv1 proton channel in human granulocytes.

Petheo GL, Orient A, Baráth M, Kovács I, Réthi B, Lányi A, Rajki A, Rajnavölgyi E, Geiszt M
PloS one 2010 Nov 23;5(11):e14081

---

NADPH oxidase contributes to coronary endothelial dysfunction in the failing heart.

Zhang P, Hou M, Li Y, Xu X, Barsoum M, Chen Y, Bache RJ
American journal of physiology. Heart and circulatory physiology 2009 Mar;296(3):H840-6

---

Enhanced amiloride-sensitive superoxide production in renal medullary thick ascending limb of Dahl salt-sensitive rats.

O'Connor PM, Lu L, Schreck C, Cowley AW Jr
American journal of physiology. Renal physiology 2008 Sep;295(3):F726-33

---

Roscovitine reduces neuronal loss, glial activation, and neurologic deficits after brain trauma.

Hilton GD, Stoica BA, Byrnes KR, Faden AI
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2008 Nov;28(11):1845-59

---

Leishmania pifanoi amastigotes avoid macrophage production of superoxide by inducing heme degradation.

Pham NK, Mouriz J, Kima PE
Infection and immunity 2005 Dec;73(12):8322-33

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 21

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.