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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-18047 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYBA Monoclonal Antibody (CS9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody is specific for human Cytochrome b245 light chain (aa 165-169, KKPSE). This target has a predicted molecular weight of 21 kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CS9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYBA/Cytochrome b245 Light Chain in human spleen cells using CYBA/Cytochrome b245 Light Chain monoclonal antibody (Product # MA5-18047).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CYBA was performed using 70% confluent log phase PC-3, DU 145 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CYBA Monoclonal Antibody (CS9) (Product # MA5-18047) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2500 dilution), for 45 minutes at room temperature (Panel a,e,i: Green). Nuclei (Panel b,f,j: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c,g,k: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d, h and i represents the merged image showing punctate staining in nucleus in both PC-3 and DU 145 but not in BJ. The images were captured at 60X magnification. CYBA localizes with Nox proteins in the nucleus in response to ROS. Between the prostate cancer cell lines, PC-3 constitutively produces higher ROS compared to DU 145 while BJ, a normal fibroblast cell line, has low endogenous levels [doi: 10.1158/0008-5472.CAN-07-5259; 10.1161/01.ATV.0000112024.13727.2c; 10.1089/ars.2008.2203]
