MA5-38098

antibody from Invitrogen Antibodies

Targeting: ZBTB33 kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, ZNF348

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38098

Provider

Invitrogen Antibodies

Product name

Kaiso Recombinant Rabbit Monoclonal Antibody (10O4V2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Samples: A-549, SH-SY5Y, Rat brain Immunogen sequence: HSQDPSGDSK LYRLHPCRSL QIRQYAYLSD RSSTIPAMKD DGIGYKVDTG KEPPVGTTTS TQNKPMTWED IFIQQENDSI FKQNVTDGST EFEFIIPESY

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

10O4V2

Vial size

100 µL

Concentration

1.04 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Kaiso in extracts of various cell lines. Samples were incubated with Kaiso Monoclonal antibody (Product # MA5-38098) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of Kaiso in HeLa cells using Kaiso Recombinant Monoclonal Antibody (Product # MA5-38098) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of Kaiso in HeLa cells using Kaiso Recombinant Monoclonal Antibody (Product # MA5-38098) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Kaiso in HeLa cells. Samples were incubated with Kaiso Monoclonal antibody (Product # MA5-38098) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Kaiso in 300 μg extracts of A-549 cells. Samples were precipitated with 3 μg Kaiso Monoclonal antibody (Product # MA5-38098). Western blot was performed from the immunoprecipitate using Kaiso Monoclonal antibody (Product # MA5-38098) at a dilution of 1:1,000.