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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-48354 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kir6.1 (KCNJ8) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Atrial Dyssynchrony Measured by Strain Echocardiography as a Marker of Proarrhythmic Remodeling and Oxidative Stress in Cardiac Surgery Patients.
Reperfusion Arrhythmias Increase after Superior Cervical Ganglionectomy Due to Conduction Disorders and Changes in Repolarization.
Sánchez FJ, Gonzalez VA, Farrando M, Baigorria Jayat AO, Segovia-Roldan M, García-Mendívil L, Ordovás L, Prado NJ, Pueyo E, Diez ER
Oxidative medicine and cellular longevity 2020;2020:8895078
Oxidative medicine and cellular longevity 2020;2020:8895078
Reperfusion Arrhythmias Increase after Superior Cervical Ganglionectomy Due to Conduction Disorders and Changes in Repolarization.
Prado NJ, Muñoz EM, Farias Altamirano LE, Aguiar F, Ponce Zumino AZ, Sánchez FJ, Miatello RM, Pueyo E, Diez ER
International journal of molecular sciences 2020 Mar 6;21(5)
International journal of molecular sciences 2020 Mar 6;21(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNJ8 in CEM cell line lysates (35 µg/lane). Lysates were probed with a KCNJ8 Antibody (N-term) (Product # PA5-48354).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of KCNJ8 in formalin fixed and paraffin embedded human heart tissue. Samples were probed with a KCNJ8 Antibody (N-term) (Product # PA5-48354) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 The pore-forming subunit (Kir6.1) of K ATP and tumor necrosis factor-alpha (TNFalpha) in SCGx rats. Kir6.1 increased in cardiomyocytes from ganglionectomized rats (yellow arrows). The levels of the inflammatory marker TNFalpha were low in ventricular samples of both groups (red arrows). Bars in the right-hand column indicate the mean integrated optical density (IOD) and SEM values relative to the level measured in the Sham group. The white scale bars in the lower right corners indicate 20 uM. In both groups, a total of 18 pictures were analyzed, six from three different hearts. * p < 0.05 vs. Sham by unpaired t-test with Welch''s correction.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Characterization of K ATP channel expression in atrial samples from synchronic and dyssynchronic patients. (a) The Kir6.1 subunit of the potassium channels was present perivascularly (white arrows) and in atrial cardiomyocytes (arrowheads). F-Actin marks the tissue with a blue staining and WGA cellular membranes to facilitate structural characterization. White bars correspond to 20 mu m. (b) Quantification of Kir6.1 integrated optical density (IOD) displayed as mean and SD and * p < 0.01 by t -test.
