MA5-27679

antibody from Invitrogen Antibodies

Targeting: KCNJ8 Kir6.1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27679

Provider

Invitrogen Antibodies

Product name

Kir6.1 (KCNJ8) Monoclonal Antibody (S366-60)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S366-60

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KCNJ8 in rat brain with 20 µg of sample. The sample was incubated with KCNJ8 monoclonal antibody (Product # MA5-27679) using a dilution of 1:1000 (16 hours at 4°C), and followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:200 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Rat Brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KCNJ8 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with KCNJ8 monoclonal antibody (Product # MA5-27679) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Kir6,1 Antibody, D) Merged image. Magnification: 60x.