A04950-1

antibody from Boster Biological Technology

Targeting: KCNJ8 Kir6.1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A04950-1

Provider

Boster Biological Technology

Product name

Anti-Kir6.1/KCNJ8 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Kir6.1/KCNJ8 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

References

Submitted references

Exogenous H2S promotes ion channel reconstruction to regulate colonic motility in rats with dinitrobenzene sulfonic acid-induced colitis.

Liu Y, Liao R, Qiang Z, Yang W, Cao J
Annals of translational medicine 2022 Jun;10(12):681

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Astragaloside IV regulates expression of ATP-sensitive potassium channel subunits after ischemia-reperfusion in rat ventricular cardiomyocytes.

Han XH, Liu P, Zhang YY, Zhang N, Chen FR, Cai JF
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2011 Dec;31(4):321-6

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Kir6.1/KCNJ8 using anti-Kir6.1/KCNJ8 antibody (A04950-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kir6.1/KCNJ8 antigen affinity purified polyclonal antibody (Catalog # A04950-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kir6.1/KCNJ8 at approximately 48KD. The expected band size for Kir6.1/KCNJ8 is at 48KD.

Additional image