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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NB600-961 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-961, RRID:AB_2178309
- Product name
- Rabbit Polyclonal RFC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RFC1 Antibody [NB600-961] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody NB600-961 (lot 2 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. RFC1 was also immunoprecipitated by prior lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
