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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-35052 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, Drosophila, C. elegans, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Loss of Adam10 Disrupts Ion Transport in Immortalized Kidney Collecting Duct Cells.
mCCD(cl1) cells show plasticity consistent with the ability to transition between principal and intercalated cells.
Assmus A, Mullins L, Ward M, Dobie R, Hunter R, Henderson NC, Mullins JJ
Function (Oxford, England) 2021;2(4):zqab024
Function (Oxford, England) 2021;2(4):zqab024
mCCD(cl1) cells show plasticity consistent with the ability to transition between principal and intercalated cells.
Assmus AM, Mansley MK, Mullins LJ, Peter A, Mullins JJ
American journal of physiology. Renal physiology 2018 May 1;314(5):F820-F831
American journal of physiology. Renal physiology 2018 May 1;314(5):F820-F831
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP6V1B1 in WiDr cells using an ATP6V1B1 polyclonal antibody (Product # PA5-35052) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ATP6V1B1 in WiDr cells. Samples were incubated in ATP6V1B1 polyclonal antibody (Product # PA5-35052) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATP6V1B1 in formalin fixed and paraffin embedded human kidney tissue. Samples were incubated with ATP6V1B1 polyclonal antibody (Product # PA5-35052) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
