Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-75580 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Roles of vacuolar H(+)-ATPase in mice treated with norepinephrine and acetylcholine.
Li Z, Zhang Y, Bickerstaff E, Yang Z, Nakamura S
International journal of clinical and experimental pathology 2020;13(6):1300-1312
International journal of clinical and experimental pathology 2020;13(6):1300-1312
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP6V1B1 in Lane 1: HEK293T cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with ATP6V1B1 polyclonal antibody (Product # PA5-75580) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-V-ATPase B1 Polyclonal Antibody (Product # PA5-75580) and a 57 kDa band corresponding to an isoform of ATP56V1B1 was observed in tissues tested except Mouse Skeletal Muscle which is reported to be negative. Tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1), Mouse Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Kidney (Lane 4) and Rat Heart (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).