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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-21003 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-21003, RRID:AB_2200557
- Product name
- Anti-TBX21
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Dual function of polycomb group proteins in differentiated murine T helper (CD4+) cells.
CCCTC-binding factor and the transcription factor T-bet orchestrate T helper 1 cell-specific structure and function at the interferon-gamma locus.
T-bet dependent removal of Sin3A-histone deacetylase complexes at the Ifng locus drives Th1 differentiation.
Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes.
Jacob E, Hod-Dvorai R, Ben-Mordechai OL, Boyko Y, Avni O
Journal of molecular signaling 2011 May 30;6:5
Journal of molecular signaling 2011 May 30;6:5
CCCTC-binding factor and the transcription factor T-bet orchestrate T helper 1 cell-specific structure and function at the interferon-gamma locus.
Sekimata M, Pérez-Melgosa M, Miller SA, Weinmann AS, Sabo PJ, Sandstrom R, Dorschner MO, Stamatoyannopoulos JA, Wilson CB
Immunity 2009 Oct 16;31(4):551-64
Immunity 2009 Oct 16;31(4):551-64
T-bet dependent removal of Sin3A-histone deacetylase complexes at the Ifng locus drives Th1 differentiation.
Chang S, Collins PL, Aune TM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8372-81
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8372-81
Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes.
Chen GY, Osada H, Santamaria-Babi LF, Kannagi R
Proceedings of the National Academy of Sciences of the United States of America 2006 Nov 7;103(45):16894-9
Proceedings of the National Academy of Sciences of the United States of America 2006 Nov 7;103(45):16894-9
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 58
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human tonsil shows strong nuclear positivity in lymphoid cells outside reaction centra.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
