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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- IC5385F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal T-bet/TBX21 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human T-bet.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis.
Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes.
Costa SF, Gomes VO, Dos Santos Maciel MO, Melo LM, Venturin GL, Bragato JP, Rebech GT, de Oliveira Santos C, Nascimento de Oliveira BM, Gileno de Sá Oliveira G, Felix de Lima VM
PLoS neglected tropical diseases 2020 Jan;14(1):e0008021
PLoS neglected tropical diseases 2020 Jan;14(1):e0008021
Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes.
Melo LM, Bragato JP, Venturin GL, Rebech GT, Costa SF, Garcia LE, Lopes FL, Eugênio FR, Patto Dos Santos PS, de Lima VMF
PloS one 2019;14(12):e0226192
PloS one 2019;14(12):e0226192
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of T-bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human T-bet/TBX21 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC5385F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
