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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 644828 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#644828, RRID:AB_2565677
- Product name
- PE/Dazzle™ 594 anti-T-bet
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Red dye
- Antibody clone number
- 4B10
- Vial size
- 100 µg
- Concentration
- 0.2 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).
